Anisotropia de fluorescência da sonda AHBA em lipossomas de misturas lipídicas e contendo colesterol

Authors

  • Bruna Eduarda Darolt Mucke Universidade Federal de Goiás
  • Cássia Alessandra Marquezin Instituto de Física - Universidade Federal de Goiás https://orcid.org/0000-0003-3203-9105

DOI:

https://doi.org/10.29384/rbfm.2024.v18.19849001755

Keywords:

fluorescence, biophysics, liposomes, lipid mixtures, cholesterol

Abstract

Hydrophobic fluorescent probes have been widely used to study model membranes, as they present different rotational dynamics for different lipid environments, being able to monitor the fluidity of a lipid bilayer and the mechanisms dependent on this factor. However, the effectiveness of using amphipathic fluorescent probes must be evaluated since the location of these probes in the membrane can cause failure in monitoring the packaging of the fatty chains. In this work, steady state fluorescence results from the AHBA probe (2-Amino-N-hexadecylbenzamide) were presented; AHBA was used to monitor the phase behavior of lipid vesicles (liposomes) formed by lipid mixtures and containing cholesterol. In the production of liposomes, the length of the hydrocarbon chain of lipids was varied, as well as the type of polar head: phosphatidylcholine (PC) and phosphatidylglycerol (PG). Parameters such as fluorescence intensity, spectral shift and static anisotropy were obtained for AHBA, inserted into these several lipid bilayers. Static anisotropy proved to be the most appropriate parameter to monitor the transition from the gel phase to the fluid phase of the bilayer, also detecting the presence of cholesterol in the system. The spectral shift results showed that AHBA has low sensitivity to discrete polarity changes in the surrounding environment.

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Published

2024-02-23

How to Cite

Darolt Mucke, B. E., & Marquezin, C. A. (2024). Anisotropia de fluorescência da sonda AHBA em lipossomas de misturas lipídicas e contendo colesterol. Brazilian Journal of Medical Physics, 18, 755. https://doi.org/10.29384/rbfm.2024.v18.19849001755

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Artigo Original